Thames Valley Cytology Society

Volume 3 Issue 1
January 2000

Joint Summer Meeting at St. Margaret's Hospital, Epping on 15th. July 1999
Report by Mark Terry, St Mary's Hospital, Paddinton

 

On Thursday 15th. of July a unique event took place with a Joint Summer Meeting between the T.V.C.S. and the Northeast Metropolitan & East Anglian Cytology Society, held at St. Margaret's Hospital, Epping.
This was the result of much effort, in particular by Meetings Secretary Hasit Patel, who not only managed to organise these joint arrangements, but put together a meetings programme of exceptional calibre, in spite of contending with unfortunate last minute changes to the line-up!
A rather long, arduous walk up-hill from the station was rewarded by a veritable banquet of home-cooked food, a capacity crowd of eager cytologists, and an exciting afternoon of lectures.

Borderline Cytology
The first talk, entitled 'Borderline Cytology, was presented by Dr Alistair Deery. He discussed the history of borderline changes from past 'inflammatory atypias', through the 1986 BSCC recommendations on recognising 'borderline nuclear abnormality', to the 1994 Joint Working Party's revision: 'Borderline nuclear changes in cervical smears: Guidelines on recognition and management'.
In particular, the problems of recognising these cellular changes were dealt with, as currently it was considered that the borderline category was open to abuse, being used as a useful place in which to place all cases that pose a particular diagnostic dilemma to the cytologist.
Dr Deery emphasised that whenever dyskaryotic changes are recognised then they must be reported as such, even if a grading as to severity can not be made. He demonstrated this point by showing a series of cases which he considered to show features of dyskaryosis but, following the 1994 Working Party's guidelines, could be classified as borderline.
Dr Deery also emphasised that more careful consideration should be given to glandular abnormalities which again may be carelessly placed into a borderline category when a diagnosis of tubal metaplasia or even true glandular neoplasia could have been made.
The recognition of true borderline changes still obviously poses a diagnostic challenge to the cytologist, and the inclusion of any case into this category should only be made after careful exclusion from other dysaryotic or benign categories.

HPV Testing
The second speaker, Dr F. Lorenzato from the Whittington Hospital, gave an informative talk on 'HPV testing and it's implications for screening'. Dr Lorenzato gave a brief history of cervical screening and the role of HPV in the aetiology of carcinogenesis, particularly as recognised since the advent of PCR testing in 1985. Identification of HPV subtypes has allowed the identification of high-risk HPV types such as 16,18,31,33, etc. and these may yet prove beneficial in cervical screening.
Identification of patients as carriers of these sub-types would allow them to be placed into a category requiring more frequent screening, particularly if these patients also presented with borderline changes in their smears. However it was emphasised that commercially available assays for HPV typing, based on hybrid capture techniques, are currently prohibitively expensive for routine use.
Vaccines developed against the E7 antigenic site on the HPV genome, responsible for blocking major protein synthesis sites such as that for P53, might also, in the future, help in reducing the incidence of HPV in the general population.

Quality Assurance
Dr Philip Wilson was next to talk, speaking of his new challenge as Q.A. Director for the N.H.S.C.S.P. in London. Following a brief history of quality assurance initiatives within cytology, from initial proficiency testing schemes to the establishment of a National Co-ordinating Network, and the later formation of the National Office, Dr Wilson brought us up to date with the latest developments.
A quality assurance team for gynaecological cytology, covering the London area, has now been established. This has led to the appointment of a Q.A. Director and a Q.A co-ordinator, the development of a Q.A reference centre, and the establishment of Q.A. groups with professional lead representatives from Cytology and Histology laboratories, Colposcopy units, primary care establishments, and Health Authorities.
The co-ordinated efforts from all these groups within the Q.A. team will no doubt provide better primary support for quality control management, the development of more comprehensive training programmes, and the careful monitoring of performance at all levels, whilst also acting as a first point of reference to all those involved in the screening programme.

ABC2 Guidelines
An excellent meeting was finally rounded off by Dr Amanda Herbert, who's talk, entitled 'ABC 2: What are the controversies', had been eagerly awaited. Dr Herbert concentrated mainly on the controversies that had arisen from the 1995 ABC guidelines and how these had been re-addressed in the updated ABC 2 guidelines ( still awaiting publication ).

Three main areas of controversy had been raised:
1. The significance of transformation zone sampling was discussed.
Cytological identification of the presence or absence of endocervical cells and/or squamous metaplastic cells was considered not to be a reliable indicator of TZ sampling but, should be used in conjunction with other pertinent patient and clinical factors in assessing smear adequacy; only the smear taker can make a true assessment of complete TZ sampling.
2. The achievable target ranges for the reporting of each grade of cytological abnormality was re-assessed.
For the 1995 guidelines, these target ranges had been based on figures obtained from only 12 screening laboratories. For ABC 2, figures have been taken from all KC61 returns for all cytology
laboratories within the U.K. In spite of this, the revised achievable standard ranges were shown to be very similar to those previously calculated with ranges only being increased slightly:
moderate & severe: 1 to 2%;
mild & borderline: 4 to 9.2 %;
inadequate: 5 to 12.7%
3. The importance of calculation for sensitivity, specificity, and positive predicative values, both for laboratories and individuals, was re-emphasised.
It was also noted that the formula for calculating sensitivity of primary screening, given in the 1995 guidelines, was inaccurate and had been updated accordingly. Dr Herbert also said that ABC 2 would also re-address the correlation between B.S.C.C. and Bethesda terminologies, whilst she also hinted that the ABC guidelines may also hold a couple of further 'surprises' We continue to wait to see what these may be!
So concluded another extremely enjoyable and informative T.V.C.S. meeting, with thanks to our colleagues at the Northeast Metropolitan & East Anglian Cytology Society, to Leica for their sponsorship,...and to Heinz baked beans who appeared in more than one speaker's presentation!